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KMID : 0613820130230030415
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Journal of Life Science
2013 Volume.23 No. 3 p.415 ~ p.425
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Physiological and Biochemical Characterization of Bacillus spp. from Polychaete, Perinereis aibuhitensis
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Shin Se-Yeon
Yundendorj Khorloo
Lee Sang-Suk
Kang Kyoung-Ho
Kahng Hyung-Yeel
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Abstract
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This study compared the characteristics of five Bacillus strains capable of aerobic and anaerobic growth, CBW3, CBW4, CBW9, CBW14 and EBW10. They were isolated and selected from a polychaete, Perinereis aibuhitensis, which is known as a good degrader of organic compounds in marine wetland. Based on a 16S rRNA sequence, CBW3 and CBW14 were found to share more than 99.8% similarity with B. nanhaiensis, B. arsenicus and B. barbaricus. CBW4, CBW9 and EBW10 shared 92.7%, 99.8%, and 99.8% similarity with B. anthracis, B. algicoa and B. thuringiensis, respectively. The temperature, salinity, and pH ranges of the cell growth of the Bacillus strains were 4-45℃, 0-17%, and pH 5-pH 9, respectively. All Bacillus strains were found to exhibit enzyme activities for the degradation of casein and starch. Notably, strain EBW10 exhibited the enzyme activities for all the tested macromolecules, DNA, casein, starch, cellulose, and four kinds of Tweens, which suggests the possibility that it had protease, amylase, cellulose, and lipase. All five Bacillus strains had alkaline phosphatase activities, and the strains CBW3, CBW4, and EBW10 also had acid phospatase. Strains CBW3 and EBW10 exhibited the enzyme activities both for esterase (C4) and esterase lipase (C8). The analysis of fatty acids revealed that in all strains, major fatty acids were anteiso C15:0 and iso C15:0.
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KEYWORD
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Bacillus spp., biodegradation, enzyme activity, marine wetland, Perinereis aibuhitensis
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